MULTI SCIENCES
Human MMP-9 Assay ELISA Kit
Human MMP-9 Assay ELISA Kit
SKU:EK1M09
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK1M09 |
| Product Name | Human MMP-9 ELISA Kit |
| Customs Name | Human MMP-9 ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 100 μl (prediluted) |
| Sensitivity | 1.70 pg/ml |
| Standard Curve Range | 156.25 - 10000 pg/ml |
| Spike Recovery Range | 85 % - 108 % |
| Mean Spike Recovery | 0.97 |
| CV of Intra plate | 3.9 % - 5.7 % |
| CV of Inter plate | 5.8 % - 7.8 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against MMP-9 Human MMP-9 Standard, lyophilized MMP-9 Detect Antibody Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human MMP-9. The Human MMP-9 ELISA is for research use only. Not for diagnostic or therapeutic procedures. Matrix metallopeptidase 9 (MMP-9), also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB), is a matrixin, a class of enzymes that belong to the zinc-metalloproteinases family involved in the degradation of the extracellular matrix. In humans the MMP9 gene encodes for a signal peptide, a propeptide, a catalytic domain with inserted three repeats of fibronectin type II domain followed by a C-terminal hemopexin-like domain. Thrombospondins, intervertebral disc proteins, regulate the effective levels of MMP-2 and -9, which are key effectors of ECM remodeling. MMP-9 has been found to be associated with numerous pathological processes, including cancer, immunologic and cardiovascular diseases. This kit is designed to measure MMP-9 (92 kDa pro- and 82 kDa active forms but not the 65 kDa form). |
