MULTI SCIENCES
Human/Mouse/Rat TGF-B2 Antibody ELISA Kit
Human/Mouse/Rat TGF-B2 Antibody ELISA Kit
SKU:EK9162
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK9162 |
| Product Name | Human/Mouse/Rat TGF-β2 ELISA Kit |
| Customs Name | Human/Mouse/Rat TGF-β2 ELISA Kit |
| Application | ELISA |
| Reactivity | Human /Mouse/Rat |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 20 μl (prediluted) |
| Sensitivity | 0.33 pg/ml |
| Standard Curve Range | 31.25 - 2000 pg/ml |
| Spike Recovery Range | 88 % - 120% |
| Mean Spike Recovery | 1.07 |
| CV of Intra plate | 2.7 % - 4.5 % |
| CV of Inter plate | 3.3 % - 4.8% |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against TGF-β2 Human/Mouse/Rat TGF-β2 Standard, lyophilized TGF-β2 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human, mouse and rat TGF-β2. The Human/Mouse/Rat TGF-β2 ELISA is for research use only. Not for diagnostic or therapeutic procedures. Transforming growth factor beta 2 (TGF-β2) is synthesized as a prepro-cytokine with a 19 amino acid (aa) signal sequence, a 283 aa pro-region, and a 112 aa mature segment. The mature region of human TGF-β2 is 71 % and 80 % identical with human TGF-β1 and TGF-β3 and 97 % identical with the corresponding mouse protein. It is expressed by a variety of cells, including osteoclasts, thymic epithelium, keratinocytes, hepatocytes, chief cells of the stomach, satellite cells, etc. TGF-β2 has marked cross-species bioactivity (e.g., human TGF-β2 is active on mouse cells, while porcine TGF-β2 is active on rabbit cells). TGF-β2 has four fundamental activities: it is a growth inhibitor for most types of cells; it enhances the deposition of extracellular matrix; it is immunosuppressive, suppressing APC expression of both IL-12 and CD40L while upregulating IL-10 secretion; and during fetal development, it is expressed in discrete areas, such as epithelium, myocardium, cartilage and bone of extremities and in the nervous system, suggesting specific functions. |
