MultiSciences

70-EK1133

Human myeloperoxidase/MPO ELISA Kit

Human myeloperoxidase/MPO ELISA Kit

ELISA

Human

Sandwich ELISA

serum, plasma, cell culture supernates

96-well strip plate

4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)

4℃

100 μl

28.72 pg/ml

62.50 - 4000 pg/ml

80 % - 122 %

1.04

1.4 % - 5.4 %

2.9 % - 5.4 %

96-well polystyrene microplate coated with a monoclonal antibody against MPO

Human MPO Standard, lyophilized

MPO Detect Antibody

Streptavidin-HRP

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

Plate Covers

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human MPO. The Human myeloperoxidase/MPO ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Myeloperoxidase (MPO) is a peroxidase enzyme most abundantly expressed in neutrophil granulocytes, and produces hypohalous acids to carry out their antimicrobial activity. MPO oxidation of apoA-I reduces HDL-mediated inhibition of apoptosis and inflammation. MPO mediates protein nitrosylation and the formation of 3-chlorotyrosine and dityrosine crosslinks.

MPO deficiency is a hereditary deficiency of the enzyme, which predisposes to immune deficiency. Studies have reported an association between elevated MPO levels and the severity of coronary artery disease. It has also been suggested that MPO plays a significant role in the development of the atherosclerotic lesion and rendering plaques unstable.