MULTI SCIENCES
Human Notch-1 ELISA Kit
Human Notch-1 ELISA Kit
SKU:EK1364
Product Details
| Factory Name | MultiSciences |
|---|---|
| Product Code | 70-EK1364-96 |
| CatNum | EK1364-96 |
| Product Name | Human Notch-1 ELISA Kit |
| Customs Name | Human Notch-1 ELISA Kit |
| Product Spec | 96T |
| SalePrice | 3200 |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Modality | NoData |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sensitivity | 3.38 pg/ml |
| Standard Curve Range | 78.13 - 5000 pg/ml |
| Spike Recovery Range | 82 %-118 % |
| Mean Spike Recovery | 98% |
| CV of Intra plate | 2.0 % - 3.3 % |
| CV of Inter plate | 2.7 % - 4.6 % |
| Components |
96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against human FGF basic Human Notch-1 Standard, lyophilized Notch-1 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate Stop Solution Washing Buffer (20×) Adhesive Films |
| PRINCIPLE OF THE ASSAY | Human Notch-1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Notch-1 in the samples. An antibody specific for human Notch-1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for Notch-1, and Notch-1 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Notch-1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader. |
| Describtion |
The Notch signaling pathway is a ubiquitous pathway that controls a variely of processes during development. The Notch family comprises a group of highly conserved proteins that function both as cell surface receptors and direct regulators of gene transcription. It plays a significant role in the regulation of cell proliferation,apoptosis,differentiation,as well as a series of physiological and pathological processes.In addition,it has a vital effect in cancer occurrence and development.Notch-1 is a 300 kDa type 1 transmembrane glycoprotein that is one of four Notch homologues involved in developmental processes . Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. Notch-1?pathway?was?associated?with?cellular?differentiation,?proliferation,?apoptosis?and adherency. The aberrant activation of Notch-1 signaling pathway has been proven to be associated with the development and progression of cancers. |
