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Human Notch-1 ELISA Kit

Human Notch-1 ELISA Kit


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Human Notch-1 ELISA Kit

Factory Name MultiSciences
Product Code 70-EK1364-96
CatNum EK1364-96
Product Name Human Notch-1 ELISA Kit
Customs Name Human Notch-1 ELISA Kit
Product Spec 96T
SalePrice 3200
Type ELISA Kit
Application ELISA
Reactivity Human
Modality NoData
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sensitivity 3.38 pg/ml
Standard Curve Range 78.13 - 5000 pg/ml
Spike Recovery Range 82 %-118 %
Mean Spike Recovery 98%
CV of Intra plate 2.0 % - 3.3 %
CV of Inter plate 2.7 % - 4.6 %

96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against human FGF basic

Human Notch-1 Standard, lyophilized

Notch-1 Detect Antibody

Standard Diluent  


Assay Buffer (10×)


Stop Solution

Washing Buffer (20×)

Adhesive Films

PRINCIPLE OF THE ASSAY Human Notch-1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Notch-1 in the samples. An antibody specific for human Notch-1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for Notch-1, and Notch-1 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Notch-1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

The Notch signaling pathway is a ubiquitous pathway that controls a variely of processes during development. The Notch family comprises a group of highly conserved proteins that function both as cell surface receptors and direct regulators of gene transcription. It plays a significant role in the regulation of cell proliferation,apoptosis,differentiation,as well as a series of physiological and pathological processes.In addition,it has a vital effect in cancer occurrence and development.Notch-1 is a 300 kDa type 1 transmembrane glycoprotein that is one of four Notch homologues involved in developmental processes . Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions.

Notch-1?pathway?was?associated?with?cellular?differentiation,?proliferation,?apoptosis?and adherency. The aberrant activation of Notch-1 signaling pathway has been proven to be associated with the development and progression of cancers.