MultiSciences

70-EK1135

Human osteopontin/OPN ELISA Kit

Human osteopontin/OPN ELISA Kit

ELISA

Human

Sandwich ELISA

serum, plasma, cell culture supernates

96-well strip plate

4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)

4℃

100 μl

4.42 pg/ml

125 - 8000 pg/ml

75 % - 126 %

0.99

4.1 % - 5.3 %

7.2 % - 9.0 %

96-well polystyrene microplate coated with a monoclonal antibody against OPN

Human OPN Standard, lyophilized

OPN Detect Antibody

Streptavidin-HRP

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

Plate Covers

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human OPN. The Human osteopontin/OPN ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Osteopontin (OPN) (also known as BSP-1, ETA-1, SPP-1 or Ric) encoded by the SPP1 gene is expressed mainly in bone. Regulation of the OPN gene is incompletely understood. Different cell types may differ in their regulatory mechanisms of the OPN gene. OPN has biological function in biomineralization, bone remodeling, immune functions, chemotaxis, cell activation and apoptosis, OPN also is associated with increased myocyte apoptosis and myocardial dysfunction.

OPN is associated with many physiological and pathological processes including wound healing, bone turnover, tumorigenesis, inflammation, ischemia, and immune responses. Manipulation of plasma (or local) OPN levels may be useful in the treatment of autoimmune diseases, cancer metastasis, bone (and tooth) mineralization diseases, osteoporosis, and some forms of stress.