96-well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody against human PD-1

Human PD-1 Standard, lyophilized

PD-1 Detect Antibody

Standard Diluent

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

Adhesive Films 

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human β-NGF. The Human  β-NGF ELISA is for research use only. Not for diagnostic or therapeutic procedures.                  Programmed cell death protein 1, also known as PD-1 and CD279, is a protein that in humans is encoded by the PDCD1 gene. PD-1 is a cell surface receptor that belongs to the immunoglobulin superfamily and is expressed on T cells and pro-B cells. In vitro, ligation of PD-1 inhibits TCR-mediated T cell proliferation and production of IL-1, IL-4, IL-10, and IFN-γ. In addition, PD-1 ligation also inhibits BCR mediated signaling. PD-1 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases. The inhibitory effect of PD-1 is accomplished through a dual mechanism of promoting apoptosis (programmed cell death) in antigen specific T-cells in lymph nodes while simultaneously reducing apoptosis in regulatory T cells.