MULTI SCIENCES
Human PDGF-AA Enzyme Immunoassay Kit
Human PDGF-AA Enzyme Immunoassay Kit
SKU:EK1213
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK1213 |
| Product Name | Human PDGF-AA ELISA Kit |
| Customs Name | Human PDGF-AA ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 100 μl (prediluted) |
| Sensitivity | 2.47 pg/ml |
| Standard Curve Range | 15.63 - 1000 pg/ml |
| Spike Recovery Range | 90%-110% |
| Mean Spike Recovery | 101% |
| CV of Intra plate | 3.4 % - 4.9 % |
| CV of Inter plate | 3.5 % - 4.5 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against PDGF-AA Human PDGF-AA Standard, lyophilized PDGF-AA Detect Antibody Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human PDGF-AA. The Human PDGF-AA ELISA is for research use only. Not for diagnostic or therapeutic procedures. Platelet-derived growth factor (PDGF) is one of the numerous growth factors that regulate cell growth and division. It is a potent mitogen for fibroblasts, smooth muscle cells and glial cells. Though PDGF is synthesized, stored, and released by platelets upon activation, it is also produced by a plethora of cells including smooth muscle cells, activated macrophages, and endothelial cells. In both mouse and human, the PDGF signalling network consists of four ligands, PDGF A-D, and two receptors, PDGF Ralpha and PDGFRbeta. All PDGFs function as secreted, disulphide-linked homodimers, but only PDGF A and B can form functional heterodimers. PDGF plays a significant role in blood vessel formation (angiogenesis), the growth of blood vessels from already-existing blood vessel tissue. Recombinant PDGF is used in medicine to help heal chronic ulcers and in orthopedic surgery and periodontistry to treat bone loss. |
