MULTI SCIENCES
Human PDGFRA/CD140a Protein A ELISA Kit
Human PDGFRA/CD140a Protein A ELISA Kit
SKU:EK1230
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK1230 |
| Product Name | Human PDGFRA/CD140a ELISA Kit |
| Customs Name | Human PDGFRA/CD140a ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 20 μl |
| Sensitivity | 6.01 pg/ml |
| Standard Curve Range | 62.50 - 4000 pg/ml |
| Spike Recovery Range | 71 % - 107 % |
| Mean Spike Recovery | 0.92 |
| CV of Intra plate | 2.2 % - 3.9 % |
| CV of Inter plate | 4.5 % - 5.8 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against PDGFRA Human PDGFRA Standard, lyophilized PDGFRA Detect Antibody Standard Diluent Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human PDGFRA. The Human PDGFRA/CD140a ELISA is for research use only. Not for diagnostic or therapeutic procedures. Platelet-derived growth factor receptor alpha (PDGFRA), also known as CD140a, together with the structurally homolog protein PDGFRB (CD140b), are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF, and Flt-3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a kinase domain in their intracellular region. PDGFRA is expressed in oligodendrocyte progenitor cells and mesothelial cell, and binds all three ligand isoforms PDGF-AA, PDGF-BB and PDGF-AB with high affinity. PDGFRA plays an essential role in regulating proliferation, chemotaxis and migration of mesangial cells. Recent studies have indicated that PDGFRA acts as a critical mediator of signaling in testis organogenesis and Leydig cell differentiation. In knockout mice, it is suggested that PDGFRA is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes. |
