MULTI SCIENCES
Human Pentraxin 3/TSG-14 ELISA Kit Plate
Human Pentraxin 3/TSG-14 ELISA Kit Plate
SKU:EK1157
Product Details
Brand | MultiSciences |
---|---|
CatNum | 70-EK1157 |
Product Name |
Human Pentraxin 3/TSG-14 ELISA Kit |
Customs Name |
Human Pentraxin 3/TSG-14 ELISA Kit |
Application |
ELISA |
Reactivity |
Human |
Assay Type |
Sandwich ELISA |
Suitable Sample Type |
serum, plasma, cell culture supernates |
Format |
96-well strip plate |
Storage |
4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
Shipping Condition |
4℃ |
Sample Volume |
10 μl |
Sensitivity |
0.56 pg/ml |
Standard Curve Range |
31.25 - 2000 pg/ml |
Spike Recovery Range |
75 % - 117 % |
Mean Spike Recovery |
0.93 |
CV of Intra plate |
2.9 % - 6.3 % |
CV of Inter plate |
2.9 % - 3.8 % |
Components |
96-well polystyrene microplate coated with a monoclonal antibody against Pentraxin 3 Human Pentraxin 3 Standard, lyophilized Pentraxin 3 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
Describtion |
This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human Pentraxin 3. The Human Pentraxin 3/TSG-14 ELISA is for research use only. Not for diagnostic or therapeutic procedures. Pentraxin 3 also known as TNF-inducible gene 14 (TSG-14) is a member of the pentraxin superfamily characterized by cyclic multimeric structure. Pentraxin 3 is rapidly produced and released by several cell types, in particular by mononuclear phagocytes, dendritic cells (DCs), fibroblasts and endothelial cells in response to primary inflammatory signals (e.g., toll-like receptor (TLR) engagement, TNFα, IL-1β). Pentraxin 3 behaves as an acute phase response protein, as the blood levels of Pentraxin 3, low in normal conditions (about 25 ng/ml in the mouse, < 2 ng/ml in humans), increase rapidly (peaking at 6 - 8 h after induction) and dramatically (200 - 800 ng/ml) during endotoxic shock, sepsis and other inflammatory and infectious conditions, correlating with the severity of the disease. Under these conditions, Pentraxin 3 is a rapid marker for primary local activation of innate immunity and inflammation. Studies showed that Pentraxin 3 has a dual role: protection against pathogens and control of autoimmunity. |
