MULTI SCIENCES
Human PIGF High Sensitivity ELISA Kit
Human PIGF High Sensitivity ELISA Kit
SKU:EK1112HS
Product Details
| Factory Name | MultiSciences |
|---|---|
| Product Code | 70-EK1112HS-96 |
| CatNum | EK1112HS-96 |
Product Name |
Human PIGF High Sensitivity ELISA Kit |
| Product Spec | 96T |
| SalePrice | 3200 |
| Type | ELISA Kit |
Application |
ELISA |
Reactivity |
Human |
| Modality | NoData |
| Assay Type | Sandwich ELISA |
Suitable Sample Type |
serum, plasma, cell culture supernates |
Format |
96-well strip plate |
Storage |
4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
Shipping Condition |
4℃ |
| Sensitivity | 0.30pg/ml |
Standard Curve Range |
3.13- 200 pg/ml |
Spike Recovery Range |
82 %-104 % |
Mean Spike Recovery |
93% |
CV of Intra plate |
1.6 % - 3.1 % |
CV of Inter plate |
2.6 % - 3.4 % |
Components |
96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against human PIGF Human PIGF HS Standard, lyophilized PIGF HS Detect Antibody Standard Diluent Streptavidin-HRP Amplification Reagent Concentrate Amplification Diluent Assay Buffer (10×) Substrate Stop Solution Washing Buffer (20×) Adhesive Films |
| PRINCIPLE OF THE ASSAY | Human PIGF High Sensitivity ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human PIGF in the samples. A monoclonal antibody specific for human PIGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and PIGF present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for PIGF is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, amplification reagent is added to the wells. Following incubation any unbound substances is removed during a wash step and streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of PIGF bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Describtion |
Placenta growth factor (PIGF) is a member of the VEGF (vascular endothelial growth factor) sub-family - a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. The main source of PIGF during pregnancy is the placental trophoblast. PIGF is also expressed in many other tissues, including the villous trophoblast. PIGF-expression within human atherosclerotic lesions is associated with plaque inflammation and neovascular growth. In both early and late onset preeclampsia, maternal serum levels of sFlt-1 are higher and PIGF lower in women presenting with preeclampsia. |
