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Human Proinsulin ELISA Kit

Human Proinsulin ELISA Kit


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Product Details

Human Proinsulin ELISA Kit

Factory Name MultiSciences
Product Code 70-EK1289 - 96
CatNum EK1289 - 96
Product Name Human Proinsulin ELISA Kit
Customs Name Human Proinsulin ELISA Kit
Product Spec 96 T
Type ELISA Kit
Application ELISA
Reactivity Human
SDS SDS - EK1289
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 20ul
Sensitivity 20.92 pg/ml 
Standard Curve Range 93.75 - 6000pg/ml
Spike Recovery Range 92 % - 118 %
Mean Spike Recovery 105%
CV of Intra plate 4.1% - 4.8%
CV of Inter plate 4.2% - 4.9%

96-well polystyrene microplate coated with a monoclonal antibody against Proinsulin

Human Proinsulin Standard, lyophilized

Proinsulin Detect Antibody

Standard Diluent

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

Plate Covers


This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human Proinsulin has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for Proinsulin are pipetted into the wells and Proinsulin present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of Proinsulin bound in the initial step. The color development is stopped and the intensity of the color is measured.