MULTI SCIENCES
Human Proinsulin ELISA Kit
Human Proinsulin ELISA Kit
SKU:EK1289
Product Details
| Factory Name | MultiSciences |
|---|---|
| Product Code | 70-EK1289 - 96 |
| CatNum | EK1289 - 96 |
| Product Name | Human Proinsulin ELISA Kit |
| Customs Name | Human Proinsulin ELISA Kit |
| Product Spec | 96 T |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| SDS | SDS - EK1289 |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 20ul |
| Sensitivity | 20.92 pg/ml |
| Standard Curve Range | 93.75 - 6000pg/ml |
| Spike Recovery Range | 92 % - 118 % |
| Mean Spike Recovery | 105% |
| CV of Intra plate | 4.1% - 4.8% |
| CV of Inter plate | 4.2% - 4.9% |
| Components |
96-well polystyrene microplate coated with a monoclonal antibody against Proinsulin Human Proinsulin Standard, lyophilized Proinsulin Detect Antibody Standard Diluent Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human Proinsulin has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for Proinsulin are pipetted into the wells and Proinsulin present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of Proinsulin bound in the initial step. The color development is stopped and the intensity of the color is measured. |
