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Human Prolactin ELISA Kit

Human Prolactin ELISA Kit


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Product Details

Human Prolactin ELISA Kit

Factory Name MultiSciences
Product Code 70-EK1304- 96
CatNum EK1304- 96
Product Name Human Prolactin ELISA Kit
Customs Name Human Prolactin ELISA Kit
Product Spec 96 T
Type ELISA Kit
Application ELISA
Reactivity Human
SDS SDS - EK1304
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 10ul
Sensitivity 23.7 pg/ml 
Standard Curve Range 0.39 - 25pg/ml
Spike Recovery Range 85 % - 116 %
Mean Spike Recovery 99%
CV of Intra plate 2.5% - 3.5%
CV of Inter plate 4.4% - 7.6%

96-well polystyrene microplate coated with a monoclonal antibody against Prolactin

Human Prolactin Standard, lyophilized

Prolactin Detect Antibody

Standard Diluent

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

Plate Covers


This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human Prolactin has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for Prolactin are pipetted into the wells and Prolactin present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of Prolactin bound in the initial step. The color development is stopped and the intensity of the color is measured.