MULTI SCIENCES
Human Prolactin ELISA Kit
Human Prolactin ELISA Kit
SKU:EK1304
Product Details
| Factory Name | MultiSciences |
|---|---|
| Product Code | 70-EK1304- 96 |
| CatNum | EK1304- 96 |
| Product Name | Human Prolactin ELISA Kit |
| Customs Name | Human Prolactin ELISA Kit |
| Product Spec | 96 T |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| SDS | SDS - EK1304 |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 10ul |
| Sensitivity | 23.7 pg/ml |
| Standard Curve Range | 0.39 - 25pg/ml |
| Spike Recovery Range | 85 % - 116 % |
| Mean Spike Recovery | 99% |
| CV of Intra plate | 2.5% - 3.5% |
| CV of Inter plate | 4.4% - 7.6% |
| Components |
96-well polystyrene microplate coated with a monoclonal antibody against Prolactin Human Prolactin Standard, lyophilized Prolactin Detect Antibody Standard Diluent Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human Prolactin has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for Prolactin are pipetted into the wells and Prolactin present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of Prolactin bound in the initial step. The color development is stopped and the intensity of the color is measured. |
