MULTI SCIENCES
Human/Rat PDGF-BB ELISA Kit Plate
Human/Rat PDGF-BB ELISA Kit Plate
SKU:EK9137
Product Details
Brand | MultiSciences |
---|---|
CatNum | 70-EK9137 |
Product Name | Human/Rat PDGF-BB ELISA Kit |
Customs Name | Human/Rat PDGF-BB ELISA Kit |
Application | ELISA |
Reactivity | Human /Rat |
Assay Type | Sandwich ELISA |
Suitable Sample Type | serum, plasma, cell culture supernates |
Format | 96-well strip plate |
Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
Shipping Condition | 4℃ |
Sample Volume | 100 μl (prediluted) |
Sensitivity | 2.13 pg/ml |
Standard Curve Range | 31.25 - 2000 pg/ml |
Spike Recovery Range | 82 % - 116 % |
Mean Spike Recovery | 95% |
CV of Intra plate | 2.3 % - 4.5 % |
CV of Inter plate | 3.3 % - 4.9 % |
Components | 96-well polystyrene microplate coated with a monoclonal antibody against PDGF-BB Human/Rat PDGF-BB Standard, lyophilized PDGF-BB Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human and rat PDGF-BB. The Human/Rat PDGF-BB ELISA is for research use only. Not for diagnostic or therapeutic procedures. Platelet-derived growth factor (PDGF) is one of the numerous growth factors that regulate cell growth and division. In particular, it plays a significant role in blood vessel formation (angiogenesis), the growth of blood vessels from already-existing blood vessel tissue. Uncontrolled angiogenesis is a characteristic of cancer. In chemical terms, PDGF is a dimeric glycoprotein composed of two A (-AA) or two B (-BB) chains or a combination of the two (-AB). PDGF-BB is expressed by hepatocytes and nonresorbing osteoclasts, generating osteoblasts and bone formation. It is also produced by platelets, macrophages, and mast cells, and at sites of injury, promotes neutrophil and macrophage infiltration for debridement, fibroblast secretion of new ECM, and IFG-1 mediated re-epithelialization. |
