MULTI SCIENCES
Human sCD40L Enzyme Immunoassay Kit
Human sCD40L Enzyme Immunoassay Kit
SKU:EK189
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK189 |
| Product Name | Human sICAM-1/CD54 ELISA Kit |
| Customs Name | Human sICAM-1/CD54 ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 50 μl (prediluted) |
| Sensitivity | 3.81 pg/ml |
| Standard Curve Range | 31.25 - 2000 pg/ml |
| Spike Recovery Range | 99 % - 118 % |
| Mean Spike Recovery | 1.1 |
| CV of Intra plate | 6.3 % - 7.5 % |
| CV of Inter plate | 4.9 % - 6.7 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against sICAM-1 Human sICAM-1 Standard, lyophilized sICAM-1 Detect Antibody Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human sICAM-1. The Human sICAM-1 ELISA is for research use only. Not for diagnostic or therapeutic procedures. Intercellular Adhesion Molecule 1 (ICAM-1), also known as CD54, is a nearly ubiquitous transmembrane glycoprotein which is typically expressed on endothelial cells and cells of the immune system and plays a key role in leukocyte migration and activation. It binds to integrins of type CD11a/CD18, or CD11b/CD18 and is also exploited by rhinovirus as a receptor. Soluble ICAM-1 (sICAM-1) promotes angiogenesis and serves as an indicator of vascular endothelial cell activation or damage. It also functions as an inhibitor of transmembrane ICAM-1 mediated activities such as monocyte adhesion to activated endothelial cells and sensitivity of tumor cells to NK cell-mediated lysis. Monitoring of sICAM-1 levels in serum provides more detailed insights in several pathological situations such as cardiovascular disease, type 2 diabetes, organ transplant dysfunction, oxidant stress, abdominal fat mass, hypertension, liver disease, and certain malignancies. |
