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Human Serum Amyloid A1 SAA1 ELISA Kit

Human Serum Amyloid A1 SAA1 ELISA Kit


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Product Details

Human Serum Amyloid A1 SAA1 ELISA Kit

Factory Name MultiSciences
CatNum EK1280
Product Name Human Serum Amyloid A1 /SAA1 ELISA Kit
Customs Name Human Serum Amyloid A1 /SAA1 ELISA Kit
Product Spec 96 T
Type ELISA Kit
Application ELISA
Reactivity Human
SDS SDS - EK1280
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 10 μl
Sensitivity 2.45 pg/ml
Standard Curve Range 312.5 - 20000 pg/ml
Spike Recovery Range 95 % - 117 %
Mean Spike Recovery 104%
CV of Intra plate 3.5 % - 4.8 %
CV of Inter plate 4.7 % - 4.9 %

96-well polystyrene microplate coated with a monoclonal antibody against SAA1

Human SAA1 Standard, lyophilized

SAA1 Detect Antibody

Standard Diluent


Assay Buffer (10×)

Substrate (TMB)

Stop Solution

washing Buffer (20×)

Plate Covers


This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human SAA1 has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for SAA1 are pipetted into the wells and SAA1 present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, Streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of SAA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.