MULTI SCIENCES
Human Serum Amyloid A1 SAA1 ELISA Kit
Human Serum Amyloid A1 SAA1 ELISA Kit
SKU:EK1280
Product Details
| Factory Name | MultiSciences |
|---|---|
| CatNum | EK1280 |
| Product Name | Human Serum Amyloid A1 /SAA1 ELISA Kit |
| Customs Name | Human Serum Amyloid A1 /SAA1 ELISA Kit |
| Product Spec | 96 T |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| SDS | SDS - EK1280 |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 10 μl |
| Sensitivity | 2.45 pg/ml |
| Standard Curve Range | 312.5 - 20000 pg/ml |
| Spike Recovery Range | 95 % - 117 % |
| Mean Spike Recovery | 104% |
| CV of Intra plate | 3.5 % - 4.8 % |
| CV of Inter plate | 4.7 % - 4.9 % |
| Components |
96-well polystyrene microplate coated with a monoclonal antibody against SAA1 Human SAA1 Standard, lyophilized SAA1 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human SAA1 has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for SAA1 are pipetted into the wells and SAA1 present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, Streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of SAA1 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
