|Product Name||Human sVCAM-1/CD106 ELISA Kit|
|Customs Name||Human sVCAM-1/CD106 ELISA Kit|
|Assay Type||Sandwich ELISA|
|Suitable Sample Type||serum, plasma, cell culture supernates|
|Format||96-well strip plate|
|Storage||4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)|
|Sample Volume||50 μl (prediluted)|
|Standard Curve Range||31.25 - 2000 pg/ml|
|Spike Recovery Range||99 % - 111 %|
|Mean Spike Recovery||0.99|
|CV of Intra plate||4.4 % - 7.0 %|
|CV of Inter plate||2.3 % - 4.5 %|
|Components||96-well polystyrene microplate coated with a monoclonal antibody against sVCAM-1
Human sVCAM-1 Standard, lyophilized
sVCAM-1 Detect Antibody
Assay Buffer (10×)
washing Buffer (20×)
|Describtion||This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human sVCAM-1. The Human sVCAM-1 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Vascular cell adhesion molecule 1 (VCAM-1), also known as CD106), is a 100-110 kDa, type I transmembrane glycoprotein typically characterized by the presence of seven C2-type immunoglobulin (Ig) domains and functions as a cell adhesion molecule. The VCAM-1 protein mediates the adhesion of lymphocytes, monocytes, eosinophils, and basophils to vascular endothelium. VCAM-1 is an endothelial ligand for VLA-4 (Very Late Antigen-4 or α4β1) of the β1 subfamily of integrins, and for integrin α4β7. VCAM-1 expression has also been observed in other cell types. It has also been shown to interact with EZR and Moesin. Other roles proposed for VCAM-1 include the regulation of osteoclastogenesis via a cell-to-cell contact mechanism and the induction of sickle cell adherence to vascular endothelial cells during hypoxemia. It also functions in leukocyte-endothelial cell signal transduction.
Monitoring of sVCAM-1 levels in serum provides more detailed insights in several pathological situations such as atherosclerosis and rheumatoid arthritis.