MULTI SCIENCES
Human SIL-2R Alpha/CD25 Antibody ELISA Kit
Human SIL-2R Alpha/CD25 Antibody ELISA Kit
SKU:EK191
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK191 |
| Product Name | Human sIL-2R alpha/CD25 ELISA Kit |
| Customs Name | Human sIL-2R alpha/CD25 ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 50 μl |
| Sensitivity | 2.99 pg/ml |
| Standard Curve Range | 78.13 - 5000 pg/ml |
| Spike Recovery Range | 93 % - 112 % |
| Mean Spike Recovery | 1.02 |
| CV of Intra plate | 4.8 % - 7.5 % |
| CV of Inter plate | 4.4 % - 7.9 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against sIL-2Rα Human sIL-2Rα Standard, lyophilized sIL-2Rα Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human sIL-2Rα/CD25. The Human sIL-2Rα/CD25 ELISA is for research use only. Not for diagnostic or therapeutic procedures. The interleukin-2 receptor (IL-2R) is a heterotrimeric protein expressed on the surface of certain immune cells, such as lymphocytes, that binds and responds to a cytokine called IL-2. IL-2R is consist of three chains, an alpha chain (IL-2Rα, CD25), a beta chain (IL-2Rβ, CD122) and a gamma chain (IL-2Rγ, CD132). The α chain binds IL-2 with low affinity, the combination of β and γ together form a complex that binds IL-2 with intermediate affinity, primarily on memory T cells and NK cells. A model of the IL-2R complex would describe the high affinity receptor as an αβγ trimer, in which all three chains are in contact with the ligand. IL-2 and its receptor have key roles in key functions of the immune system, tolerance and immunity, primarily via their direct effects on T cells. But the function of the soluble IL-2 Rα (sIL-2Rα) is unclear, since it would be expected to be a poor inhibitor of IL-2 because of its low binding affinity. In any case, increased levels of the sIL-2Rα in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Monitoring of sIL-2Rα levels in serum provides more detailed insights in several pathological situations such as rheumatoid arthritis, systemic lupus erythematosis (SLE), some leukemias and lymphomas. |
