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MULTI SCIENCES

Human SP-D ?enzyme immunoassay Kit

Human SP-D ?enzyme immunoassay Kit

EK1166

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Product Details

Human SP-D ?enzyme immunoassay Kit

Brand

MultiSciences

Cat Num

70-EK1166

Product Name

Human SP-D ELISA Kit

Customs Name

Human SP-D ELISA Kit

Application

ELISA

Reactivity

Human

Assay Type

Sandwich ELISA

Suitable Sample Type

serum, plasma, cell culture supernates

Format

96-well strip plate

Storage

4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)

Shipping Condition

4℃

Sample Volume

10 μl

Sensitivity

2.45 pg/ml

Standard Curve Range

78.13 - 5000 pg/ml

Spike Recovery Range

94 % - 120 %

Mean Spike Recovery

1.05

CV of Intra plate

5.2 % - 6.1 %

CV of Inter plate

3.2 % - 8.0 %

Components

96-well polystyrene microplate coated with a monoclonal antibody against SP-D
Human SP-D Standard, lyophilized
SP-D Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers

Describtion

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human SP-D. The Human SP-D ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Surfactant Protein D (SP-D) is a 43 kDa member of the collectin family of innate immune modulators. It is constitutively secreted by alveolar lining cells and epithelium associated with tubular structures and induced in cardiac smooth muscle and endothelial cells. Human SP-D is found in serum, plasma, broncho-alveolar lavage fluid, and amniotic fluid.
SP-D plays an important role in host defense and innate immunity. It binds human neutrophil defensins, modulating influenza anti-viral defense. It has increasing evident that SP-D involves in the pathogenesis of many pulmonary diseases, and serves as a biomarker.