MULTI SCIENCES
Human SVCAM-1/CD106 ELISA Kit Plate
Human SVCAM-1/CD106 ELISA Kit Plate
SKU:EK190
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK190 |
| Product Name | Human sVCAM-1/CD106 ELISA Kit |
| Customs Name | Human sVCAM-1/CD106 ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 50 μl (prediluted) |
| Sensitivity | 2.65 pg/ml |
| Standard Curve Range | 31.25 - 2000 pg/ml |
| Spike Recovery Range | 99 % - 111 % |
| Mean Spike Recovery | 0.99 |
| CV of Intra plate | 4.4 % - 7.0 % |
| CV of Inter plate | 2.3 % - 4.5 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against sVCAM-1 Human sVCAM-1 Standard, lyophilized sVCAM-1 Detect Antibody Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human sVCAM-1. The Human sVCAM-1 ELISA is for research use only. Not for diagnostic or therapeutic procedures. Vascular cell adhesion molecule 1 (VCAM-1), also known as CD106), is a 100-110 kDa, type I transmembrane glycoprotein typically characterized by the presence of seven C2-type immunoglobulin (Ig) domains and functions as a cell adhesion molecule. The VCAM-1 protein mediates the adhesion of lymphocytes, monocytes, eosinophils, and basophils to vascular endothelium. VCAM-1 is an endothelial ligand for VLA-4 (Very Late Antigen-4 or α4β1) of the β1 subfamily of integrins, and for integrin α4β7. VCAM-1 expression has also been observed in other cell types. It has also been shown to interact with EZR and Moesin. Other roles proposed for VCAM-1 include the regulation of osteoclastogenesis via a cell-to-cell contact mechanism and the induction of sickle cell adherence to vascular endothelial cells during hypoxemia. It also functions in leukocyte-endothelial cell signal transduction. Monitoring of sVCAM-1 levels in serum provides more detailed insights in several pathological situations such as atherosclerosis and rheumatoid arthritis. |
