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MULTI SCIENCES

Human T-Plasminogen Activator ELISA Kit

Human T-Plasminogen Activator ELISA Kit

SKU:EK1307

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Product Details

Human T-Plasminogen Activator ELISA Kit

Factory Name MultiSciences
Product Code 70-EK1307-96
CatNum EK1307-96
Product Name Human t-Plasminogen Activator ELISA Kit
Product Spec 96 T
SalePrice 2500
Type ELISA Kit
Application ELISA
Reactivity Human
Modality NoData
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sensitivity 0.81 pg/ml
Standard Curve Range 62.5 - 4000 pg/ml
Spike Recovery Range 73 %-113 %
Mean Spike Recovery 93%
CV of Intra plate 2.4 % - 7.4 %
CV of Inter plate 3.2 % - 5.3 %
Components

96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against human tPA

Human tPA Standard, lyophilized

tPA Detect Antibody

Standard Diluent

Streptavidin-HRP                                                                                  

Assay Buffer (10×)

Substrate

Stop Solution

Washing Buffer (20×)

Adhesive Films

PRINCIPLE OF THE ASSAY Human t-Plasminogen Activator/tPA ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human tPA in the samples. A monoclonal antibody specific for human tPA has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for tPA, and tPA present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of tPA bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Describtion

Tissue Plasminogen Activator (tPA) is a serine protease that converts the zymogen Plasminogen into the active serine protease plasmin, the primary enzyme responsible for the removal of fibrin deposits. The biological effects of tPA include blood clot degradation, vascular remodeling, synaptic plasticity, and neurodegeneration in the brain following trauma. tPA is also rapidly cleared from the extracellular and vascular space through Low-Density Lipoprotein Receptor-related Protein-3 mediated endocytosis. Increased expression or activity of tPA is associated with excessive bleeding, while reduced tPA activity has been implicated in thrombosis and embolism formation. In the brain, tPA is expressed in neurons, astrocytes, microglia, and vascular parenchymal endothelial cells. Changes in tPA expression in the brain have been shown following stroke, hypoxia, excitotoxic trauma, and stress-induced cognitive decline.