MULTI SCIENCES
Human T-Plasminogen Activator ELISA Kit
Human T-Plasminogen Activator ELISA Kit
SKU:EK1307
Product Details
| Factory Name | MultiSciences |
|---|---|
| Product Code | 70-EK1307-96 |
| CatNum | EK1307-96 |
| Product Name | Human t-Plasminogen Activator ELISA Kit |
| Product Spec | 96 T |
| SalePrice | 2500 |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Modality | NoData |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sensitivity | 0.81 pg/ml |
| Standard Curve Range | 62.5 - 4000 pg/ml |
| Spike Recovery Range | 73 %-113 % |
| Mean Spike Recovery | 93% |
| CV of Intra plate | 2.4 % - 7.4 % |
| CV of Inter plate | 3.2 % - 5.3 % |
| Components |
96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against human tPA Human tPA Standard, lyophilized tPA Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate Stop Solution Washing Buffer (20×) Adhesive Films |
| PRINCIPLE OF THE ASSAY | Human t-Plasminogen Activator/tPA ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human tPA in the samples. A monoclonal antibody specific for human tPA has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for tPA, and tPA present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of tPA bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader. |
| Describtion | Tissue Plasminogen Activator (tPA) is a serine protease that converts the zymogen Plasminogen into the active serine protease plasmin, the primary enzyme responsible for the removal of fibrin deposits. The biological effects of tPA include blood clot degradation, vascular remodeling, synaptic plasticity, and neurodegeneration in the brain following trauma. tPA is also rapidly cleared from the extracellular and vascular space through Low-Density Lipoprotein Receptor-related Protein-3 mediated endocytosis. Increased expression or activity of tPA is associated with excessive bleeding, while reduced tPA activity has been implicated in thrombosis and embolism formation. In the brain, tPA is expressed in neurons, astrocytes, microglia, and vascular parenchymal endothelial cells. Changes in tPA expression in the brain have been shown following stroke, hypoxia, excitotoxic trauma, and stress-induced cognitive decline. |
