MULTI SCIENCES
Human Thioredoxin-1/Trx-1 ELISA Kit DsitriButor
Human Thioredoxin-1/Trx-1 ELISA Kit DsitriButor
SKU:EK1222
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK1222 |
| Product Name | Human Thioredoxin-1/Trx-1 ELISA Kit |
| Customs Name | Human Thioredoxin-1/Trx-1 ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 100 μl (prediluted) |
| Sensitivity | 3.92 pg/ml |
| Standard Curve Range | 46.88 - 3000 pg/ml |
| Spike Recovery Range | 81%-110% |
| Mean Spike Recovery | 100% |
| CV of Intra plate | 3.9% - 4.9% |
| CV of Inter plate | 4.9% - 5.0% |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against Trx-1 Human Trx-1 Standard, lyophilized Trx-1 Detect Antibody Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human Trx-1. The Human Thioredoxin-1/Trx-1 ELISA is for research use only. Not for diagnostic or therapeutic procedures. Thioredoxins (Trxs) are a group of small ubiquitous proteins in all living cells that are key regulators of cellular redox balance. The mammalian Trx family has three members: Trx-1, Trx-2, and TrxL. Trx-1 is the only extracellular occurring thioredoxin, and is secreted by lymphocytes, hepatocytes, fibroblasts, and several tumor cells. Plasma concentrations of Trx-1 are up to 6 nM. In cells, Trx-1 is localized predominantly in the cytoplasm. Small amounts have been detected in the nucleus and in association with the outside surface of the cells. Expression of Trx-1 is increased under various stress conditions such as hypoxia, elevated hydrogen peroxide concentrations, photochemical oxidative stress, and viral and bacterial infections. Biological functions of Trx-1 include growth factor activity, antioxidant properties, a cofactor that provides reducing equivalents, and transcriptional regulation. The synovial tissue of rheumatoid arthritis patients produces increased levels of Trx-1 under oxidative stress conditions, and a correlation exists between the plasma levels of Trx-1 and the severity of the disease, making Trx-1 a biomarker for this pathological condition. |
