MULTI SCIENCES
Human TIMP-3 ELISA Kit
Human TIMP-3 ELISA Kit
SKU:EK1284
Product Details
| Factory Name | MultiSciences |
|---|---|
| Product Code | 70-EK1284 - 96 |
| CatNum | EK1284 - 96 |
| Product Name | Human TIMP-3 ELISA Kit |
| Customs Name | Human TIMP-3 ELISA Kit |
| Product Spec | 96 T |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| SDS | SDS - EK1284 |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 100ul |
| Sensitivity | 1.74 pg/ml |
| Standard Curve Range | 15.63 - 1000 pg/ml |
| Spike Recovery Range | 97 % - 116 % |
| Mean Spike Recovery | 104% |
| CV of Intra plate | 4.3% - 4.9% |
| CV of Inter plate | 4.7% - 4.9% |
| Components |
96-well polystyrene microplate coated with a monoclonal antibody against TIMP-3 Human TIMP-3 Standard, lyophilized TIMP-3 Detect Antibody Standard Diluent Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human TIMP-3 has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for TIMP-3 are pipetted into the wells and TIMP-3 present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, Streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of TIMP-3 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
