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Human TIMP-3 ELISA Kit

Human TIMP-3 ELISA Kit


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Product Details

Human TIMP-3 ELISA Kit

Factory Name MultiSciences
Product Code 70-EK1284 - 96
CatNum EK1284 - 96
Product Name Human TIMP-3 ELISA Kit
Customs Name Human TIMP-3 ELISA Kit
Product Spec 96 T
Type ELISA Kit
Application ELISA
Reactivity Human
SDS SDS - EK1284
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 100ul
Sensitivity 1.74 pg/ml
Standard Curve Range 15.63 - 1000 pg/ml
Spike Recovery Range 97 % - 116 % 
Mean Spike Recovery 104%
CV of Intra plate 4.3% - 4.9%
CV of Inter plate 4.7% - 4.9%

96-well polystyrene microplate coated with a monoclonal antibody against TIMP-3

Human TIMP-3 Standard, lyophilized

TIMP-3 Detect Antibody

Standard Diluent

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

Plate Covers


This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human TIMP-3 has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for TIMP-3 are pipetted into the wells and TIMP-3 present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, Streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of TIMP-3 bound in the initial step. The color development is stopped and the intensity of the color is measured.