96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against human TLR2

Human TLR2 Standard, lyophilized

TLR2 Detect Antibody

Standard Diluent

Streptavidin-HRP                                                                                           

Assay Buffer (10×)

Substrate

Stop Solution

Washing Buffer (20×)

Adhesive Films

Human TLR2 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human TLR2 in the samples. An antibody specific for human TLR2 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for TLR2, and TLR2 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of TLR2 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Toll-Like Receptor 2 (TLR2) is a transmembrane receptor expressed on a wide variety of cell types including microglia, monocytes, macrophages and dendritic cells. TLRs make up a family of pattern recognition receptors that play important roles in the innate immune response.Broad classes of pathogens (e.g. viruses, bacteria, and fungi) constitutively express a set of mutation-resistant molecules called pathogen-associated molecular patterns (PAMPs). These microbial molecular markers may be composed of proteins, carbohydrates, lipids, nucleic acids and/or combinations thereof. Individual TLRs recognize distinct pathogen-associated PAMPs, initiating signaling cascades that promote the immune response.