|Product Name||Human TrkB/NTRK2 ELISA Kit|
|Assay Type||Sandwich ELISA|
|Suitable Sample Type||serum, plasma, cell culture supernates|
|Format||96-well strip plate|
|Storage||4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)|
|Sample Volume||100 μl (prediluted)|
|Standard Curve Range||15.63 - 1000 pg/ml|
|Spike Recovery Range||88%-112%
|Mean Spike Recovery||97%
|CV of Intra plate||1.9 % - 3.0 %|
|CV of Inter plate||3.3 % - 3.6 %|
|Components||96-well polystyrene microplate coated with a monoclonal antibody against TrkB
Human TrkB Standard, lyophilized
TrkB Detect Antibody
Assay Buffer (10×)
Washing Buffer (20×)
|Describtion||This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human TrkB. The Human TrkB/NTRK2 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Tropomyosin receptor kinase B (TrkB), also known as tyrosine receptor kinase B, a receptor for brain-derived neurotrophic factor (BDNF), is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor exclusively expressed in neurons of the central nervous system. TrkB mediates the multiple effects of neurotrophic factors such as BDNF, neurotrophin-4 (NT-4), and neurotrophin-3 (NT-3), which includes neuronal differentiation and survival. It has two distinct binding regions for BDNF within the extracellular domain with high affinity. Upon neurotrophin binding, TrkB phosphorylates itself and the downstream molecular members of the related signal pathways.
TrkB has been shown to play an important role in tumorigenesis and metastasis, and mutations in this gene are associated with obesity and mood disorders. Accordingly, BDNF/TrkB signaling is thought to be a potential therapeutic target for schizophrenia.