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MULTI SCIENCES

Human TSH ELISA Kit

Human TSH ELISA Kit

SKU:EK1303

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Product Details

Human TSH ELISA Kit

Factory Name MultiSciences
Product Code 70-EK1303 - 96
CatNum EK1303 - 96
Product Name Human TSH ELISA Kit
Customs Name Human TSH ELISA Kit
Product Spec 96 T
Type ELISA Kit
Application ELISA
Reactivity Human
SDS SDS - EK1303
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 50ul
Sensitivity 2.01 pg/ml 
Standard Curve Range 31.25 - 2000pg/ml
Spike Recovery Range 97 % - 118 %
Mean Spike Recovery 106%
CV of Intra plate 2.5% - 4.2%
CV of Inter plate 3.6% - 5.1%
Components

96-well polystyrene microplate coated with a monoclonal antibody against TSH

Human TSH Standard, lyophilized

TSH Detect Antibody

Standard Diluent

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

Plate Covers

Describtion

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human TSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and TSH present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for TSH is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, Streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of TSH bound in the initial step. The color development is stopped and the intensity of the color is measured.