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Human TSLP Enzyme Immunoassay Kit

Human TSLP Enzyme Immunoassay Kit


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Product Details

Human TSLP  Enzyme Immunoassay Kit

Brand MultiSciences
CatNum 70-EK165
Product Name Human TSLP ELISA Kit
Customs Name Human TSLP ELISA Kit
Application ELISA
Reactivity Human
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 20 μl
Sensitivity 0.13 pg/ml
Standard Curve Range 15.63 - 1000 pg/ml
Spike Recovery Range 85 % - 118 %
Mean Spike Recovery 1
CV of Intra plate 3.3 % - 4.1 %
CV of Inter plate 5.2 % - 7.5 %
Components 96-well polystyrene microplate coated with a monoclonal antibody against TSLP
Human TSLP Standard, lyophilized
TSLP Detect Antibody
Standard Diluent
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
Describtion This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human TSLP. The Human TSLP ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Thymic stromal lymphopoietin (TSLP) is produced mainly by non-hematopoietic cells such as fibroblasts, epithelial cells and different types of stromal or stromal-like cells. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes[citation needed] and enhances the maturation of myeloid (CD11c+) dendritic cells.TSLP has also been shown to activate the maturation of a specific subset of dendritic cells located within the epidermis, called Langerhans cells. [4] Within the thymus TSLP activation of both myeloid and plasmacytoid (CD123+) dendritic cells results in the production of regulatory T cells. TSLP signals through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor CRLF2 and the IL-7R alpha chain. After binding STAT5 phosphorylation is induced resulting in the expression of upstream transcription factors. TSLP expression is linked to many disease states including asthma, inflammatory arthritis, atopic dermatitis, and eczema and other allergic states.