MULTI SCIENCES
Human TSLP Enzyme Immunoassay Kit
Human TSLP Enzyme Immunoassay Kit
SKU:EK165
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK165 |
| Product Name | Human TSLP ELISA Kit |
| Customs Name | Human TSLP ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 20 μl |
| Sensitivity | 0.13 pg/ml |
| Standard Curve Range | 15.63 - 1000 pg/ml |
| Spike Recovery Range | 85 % - 118 % |
| Mean Spike Recovery | 1 |
| CV of Intra plate | 3.3 % - 4.1 % |
| CV of Inter plate | 5.2 % - 7.5 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against TSLP Human TSLP Standard, lyophilized TSLP Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human TSLP. The Human TSLP ELISA is for research use only. Not for diagnostic or therapeutic procedures. Thymic stromal lymphopoietin (TSLP) is produced mainly by non-hematopoietic cells such as fibroblasts, epithelial cells and different types of stromal or stromal-like cells. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes[citation needed] and enhances the maturation of myeloid (CD11c+) dendritic cells.TSLP has also been shown to activate the maturation of a specific subset of dendritic cells located within the epidermis, called Langerhans cells. [4] Within the thymus TSLP activation of both myeloid and plasmacytoid (CD123+) dendritic cells results in the production of regulatory T cells. TSLP signals through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor CRLF2 and the IL-7R alpha chain. After binding STAT5 phosphorylation is induced resulting in the expression of upstream transcription factors. TSLP expression is linked to many disease states including asthma, inflammatory arthritis, atopic dermatitis, and eczema and other allergic states. |
