MultiSciences

70-EK1159

Human VEGF R2/KDR ELISA Kit

Human VEGF R2/KDR ELISA Kit

ELISA

Human

Sandwich ELISA

serum, plasma, cell culture supernates

96-well strip plate

4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)

4℃

100 μl (prediluted)

0.52 pg/ml

62.50 - 4000 pg/ml

88 % - 107 %

0.94

3.7 % - 4.0 %

3.6 % - 3.9 %

96-well polystyrene microplate coated with a monoclonal antibody against VEGF R2
Human VEGF R2 Standard, lyophilized
VEGF R2 Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human VEGF R2. The Human VEGF R2/KDR ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Vascular endothelial growth factor receptor-2 (VEGF R2), also known as kinase insert domain receptor (KDR), is a member of the class III subfamily of receptor tyrosine kinases (RTKs). VEGF R2 is more widely distributed and expressed in all vessel-derived endothelial cells in comparison to VEGF R1. VEGF R2 binds VEGF-A (VEGF121, VEGF165, VEGF189 and VEGF206 splice variants), VEGF-C and VEGF-D. Soluble forms of VEGF R1 and VEGF R2 differ significantly from one another in terms of their abilities to block VEGF-induced cell proliferation and migration. Soluble VEGF R2 cannot compete with VEGF for binding to human endothelial cells expressing both VEGF R1 and VEGF R2, in contrast to soluble VEGF R1. Soluble VEGF R2 can only partially inhibit cell migration, whereas soluble VEGF R1 can almost completely block VEGF-induced cell proliferation and migration. The binding of VEGF to soluble VEGF R2, but not VEGF R1, is also dependent on heparin. The VEGF/VEGF R2 signaling pathway plays an important role in tumor angiogenesis and other diseases where "pathological angiogenesis" is involved. Inactivation of functional VEGF R2 by a blocking antibody can disrupt angiogenesis and prevent tumor cell invasion.