MULTI SCIENCES
Human Von WilleBrand Factor ELISA Kit Plate
Human Von WilleBrand Factor ELISA Kit Plate
SKU:EK192
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK192 |
| Product Name | Human von Willebrand Factor ELISA Kit |
| Customs Name | Human von Willebrand Factor ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 100μl (Prediluted) |
| Sensitivity | 0.47 pg/ml |
| Standard Curve Range | 156.25 - 10000 pg/ml |
| Spike Recovery Range | 92 % - 110 % |
| Mean Spike Recovery | 1.01 |
| CV of Intra plate | 3.7 % - 5.5 % |
| CV of Inter plate | 2.4 % - 6.1 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against vWF Human vWF Standard, lyophilized vWF Detect Antibody Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human vWF. The Human vWF ELISA is for research use only. Not for diagnostic or therapeutic procedures. Von Willebrand factor (vWF) is a blood multimeric glycoprotein present in blood plasma and produced constitutively as ultra-large vWF in endothelium (in the Weibel-Palade bodies), megakaryocytes (α-granules of platelets), and subendothelial connective tissue. Multimers of vWF can be extremely large, >20,000 kDa, and consist of over 80 subunits of 250 kDa each. Every monomer of vWF contains a number of specific domains with a specific function, elements of note are D'/D3 domain, A1 domain, A3 domain, C1 domain and the "cysteine knot" domain. The primary function of vWF is binding to other proteins, in particular factor VIII, and it is important in platelet adhesion to wound sites. vWF plays a major role in blood coagulation. Therefore, vWF deficiency or dysfunction (von Willebrand disease) leads to a bleeding tendency, which is most apparent in tissues having high blood flow shear in narrow vessels. Monitoring of vWF levels in serum provides more detailed insights in several pathological situations such as thrombotic thrombocytopenic purpura, Heyde's syndrome, and possibly hemolytic-uremic syndrome. |
