96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against mouse BAFF

Mouse BAFF Standard, lyophilized

BAFF Detect Antibody

Standard Diluent

Streptavidin-HRP                                                                                           

Assay Buffer (10×)

Substrate

Stop Solution

Washing Buffer (20×)

Adhesive Films

Mouse BAFF/BLyS/TNFSF13B ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse BAFF in the samples. An antibody specific for mouse BAFF has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for BAFF, and BAFF present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of BAFF bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

B-cell activating factor (BAFF), also known as B Lymphocyte Stimulator (BLyS) or tumor necrosis factor ligand superfamily member 13B (TNFSF13B), is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. 

As an immunostimulant, BAFF is necessary for maintaining normal immunity. Inadequate level of BAFF will fail to activate B cells to produce enough immunoglobulin and will lead to immunodeficiency. Excessive level of BAFF causes abnormally high antibody production, results in systemic lupus erythmatosis, rheumatoid arthritis, and many other autoimmune diseases.