MULTI SCIENCES
Mouse CCL4/MIP-1B Sandwich ELISA Kit
Mouse CCL4/MIP-1B Sandwich ELISA Kit
SKU:EK262
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK262 |
| Product Name | Mouse CCL4/MIP-1β ELISA Kit |
| Customs Name | Mouse CCL4/MIP-1β ELISA Kit |
| Application | ELISA |
| Reactivity | Mouse |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 20 μl |
| Sensitivity | 0.84 pg/ml |
| Standard Curve Range | 62.50 - 4000 pg/ml |
| Spike Recovery Range | 70 % - 106 % |
| Mean Spike Recovery | 0.88 |
| CV of Intra plate | 4.4 % - 5.7 % |
| CV of Inter plate | 3.4 % - 4.8 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against CCL4 Mouse CCL4 Standard, lyophilized CCL4 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse CCL4. The Mouse CCL4/MIP-1β ELISA is for research use only. Not for diagnostic or therapeutic procedures. The chemokine (C-C motif) ligand 4 (CCL4), also known as Macrophage inflammatory protein 1β (MIP-1β), is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 expression can be induced in a variety of mouse cell types, including mast cells, endothelial cells, macrophages and CD8+ T cells. Mouse CCL4 cDNA encodes a 92 amino acid (aa) residue precursor protein with a 23 aa residue signal peptide that is cleaved to generate the 69 aa residue secreted mature protein. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Chemokine activities are mediated by G-protein-coupled seven transmembrane domain receptors. These receptors, CCR1, 5, and 8 have been shown to mediate the biological activities of CCL4. CCL4 was reported to be twenty-fold less active than CCL3 on the same cell populations. |
