|Product Name||Mouse Dkk-1 ELISA Kit|
|Customs Name||Mouse Dkk-1 ELISA Kit|
|Assay Type||Sandwich ELISA|
|Suitable Sample Type||serum, plasma, cell culture supernates|
|Format||96-well strip plate|
|Storage||4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)|
|Sample Volume||100 μl (prediluted)|
|Standard Curve Range||93.75 - 6000 pg/ml|
|Spike Recovery Range||80 % - 118 %|
|Mean Spike Recovery||1%|
|CV of Intra plate||2.7 % - 4.2 %|
|CV of Inter plate||2.5 % - 4.2 %|
|Components||96-well polystyrene microplate coated with a monoclonal antibody against Dkk-1
Mouse Dkk-1 Standard, lyophilized
Dkk-1 Detect Antibody
Assay Buffer (10×)
Washing Buffer (20×)
|Describtion||This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse Dkk-1. The Mouse Dkk-1 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Dickkopf related protein 1 (Dkk-1) is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the Wnt signaling pathway. All mice that were homozygous for the Dkk-1 knockout were dead at birth due to defects in the cranium and structures formed by the neural crest, such as failed development of eyes, olfactory placodes, frontonasal mass and mandibular processes, as well as incomplete development of the forebrain and midbrain and fusion of the digits of the forelimb.
Elevated levels of Dkk-1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.