|Product Name||Mouse E-Cadherin ELISA Kit|
|Customs Name||Mouse E-Cadherin ELISA Kit|
|Assay Type||Sandwich ELISA|
|Suitable Sample Type||serum, plasma, cell culture supernates|
|Format||96-well strip plate|
|Storage||4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)|
|Standard Curve Range||46.88 - 3000 pg/ml|
|Spike Recovery Range||95 %-110 %|
|Mean Spike Recovery||99%|
|CV of Intra plate||3.0 % - 4.8 %|
|CV of Inter plate||3.4 % - 4.8 %|
96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against mouse E-Cadherin
Mouse E-Cadherin Standard, lyophilized
E-Cadherin Detect Antibody
Assay Buffer (10×)
Washing Buffer (20×)
|PRINCIPLE OF THE ASSAY||
Mouse E-Cadherin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse E-Cadherin in the samples. An antibody specific for mouse E-Cadherin has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for E-Cadherin, and E-Cadherin present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of E-Cadherin bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
E-Cadherin, also known as Cadherin-1, is a 120 kDa member of the Cadherin family of cell surface glycoproteins that mediate cell adhesion. Classical cadherins contain five extracellular cadherin repeats and a conserved cytoplasmic region that interacts with the actin cytoskeleton via catenins. In addition to cell adhesion, cadherins also mediate morphogenesis, cytoskeletal organization, and cell migration.
E-Cadherin is a single-pass transmembrane protein on the plasma membrane. E-Cadherin is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. E-Cadherin expression is critical for epithelial tissue homeostasis. Decreased E-Cadherin is associated with physiological and pathological epithelial-to-mesenchymal transition and cell migration, and E-Cadherin loss contributes to cancer metastasis. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity.