MULTI SCIENCES
Mouse E-Cadherin ELISA Kit
Mouse E-Cadherin ELISA Kit
SKU:EK2342
Product Details
Factory Name | MultiSciences |
---|---|
Product Code | 70-EK2342-96 |
CatNum | EK2342-96 |
Product Name | Mouse E-Cadherin ELISA Kit |
Customs Name | Mouse E-Cadherin ELISA Kit |
Product Spec | 96T |
SalePrice | 3200 |
Type | ELISA Kit |
Application | ELISA |
Reactivity | Mouse |
Modality | NoData |
Assay Type | Sandwich ELISA |
Suitable Sample Type | serum, plasma, cell culture supernates |
Format | 96-well strip plate |
Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
Shipping Condition | 4℃ |
Sensitivity | 1.08 pg/ml |
Standard Curve Range | 46.88 - 3000 pg/ml |
Spike Recovery Range | 95 %-110 % |
Mean Spike Recovery | 99% |
CV of Intra plate | 3.0 % - 4.8 % |
CV of Inter plate | 3.4 % - 4.8 % |
Components |
96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against mouse E-Cadherin Mouse E-Cadherin Standard, lyophilized E-Cadherin Detect Antibody Streptavidin-HRP Assay Buffer (10×) Substrate Stop Solution Washing Buffer (20×) Adhesive Films |
PRINCIPLE OF THE ASSAY | Mouse E-Cadherin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse E-Cadherin in the samples. An antibody specific for mouse E-Cadherin has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for E-Cadherin, and E-Cadherin present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of E-Cadherin bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader. |
Describtion |
E-Cadherin, also known as Cadherin-1, is a 120 kDa member of the Cadherin family of cell surface glycoproteins that mediate cell adhesion. Classical cadherins contain five extracellular cadherin repeats and a conserved cytoplasmic region that interacts with the actin cytoskeleton via catenins. In addition to cell adhesion, cadherins also mediate morphogenesis, cytoskeletal organization, and cell migration. E-Cadherin is a single-pass transmembrane protein on the plasma membrane. E-Cadherin is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. E-Cadherin expression is critical for epithelial tissue homeostasis. Decreased E-Cadherin is associated with physiological and pathological epithelial-to-mesenchymal transition and cell migration, and E-Cadherin loss contributes to cancer metastasis. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. |
