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Mouse Erythropoietin/EPO ELISA Kit

Mouse Erythropoietin/EPO ELISA Kit


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Mouse Erythropoietin/EPO ELISA Kit

Factory Name MultiSciences
Product Code 70-EK2E01-96
CatNum EK2E01-96
Product Name Mouse Erythropoietin/EPO ELISA Kit
Customs Name Mouse Erythropoietin/EPO ELISA Kit
Product Spec 96T
SalePrice 3200
Type ELISA Kit
Application ELISA
Reactivity Mouse
Modality NoData
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sensitivity 2.91 pg/ml
Standard Curve Range 31.25 - 2000 pg/ml
Spike Recovery Range 83 %-125 %
Mean Spike Recovery 108%
CV of Intra plate 2.3 % - 4.1 %
CV of Inter plate 3.9 % - 5.1 %

96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against mouse EPO

Mouse EPO Standard, lyophilized

EPO Detect Antibody


Assay Buffer (10×)


Stop Solution

Washing Buffer (20×)

Adhesive Films


Mouse Erythropoietin/EPO ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse EPO in the samples. An antibody specific for mouse EPO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and EPO present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for EPO is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of EPO bound

 in the initial step. The color development is stopped and the intensity of the color is measured.


Erythropoietin (EPO) is a 34-39 kDa secreted glycoprotein that is a member of the type I cytokine superfamily. EPO is primarily produced by cells in the kidney (interstitial peritubular renal fibroblasts) and liver (hepatocytes and Ito cells), where its production is up-regulated by hypoxia. Erythropoietin is the principal hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Mouse Erythropoietin has 94% amino acids sequence identity to rat Erythropoietin, and 80% identity to the human Erythropoietin. Apart from its role in erythropoiesis, EPO also acts on various non-hematopoietic cells to function as a viability and proliferation factor. EPO can stimulate myoblast proliferation while suppressing its differentiation, resulting in the expansion of the progenitor cell population. EPO is a tissueprotective factor that protects against ischemic and toxic injuries to neuronal, cardiovascular and renal tissues.