MULTI SCIENCES
Mouse FGF Acidic ELISA Kit
Mouse FGF Acidic ELISA Kit
SKU:EK2285
Product Details
| Brand | MultiSciences |
|---|---|
| Product Code | 70-EK2285 - 96 |
| CatNum | EK2285 - 96 |
| Product Name | Mouse FGF acidic ELISA Kit |
| Customs Name | Mouse FGF acidic ELISA Kit |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Mouse |
| SDS | SDS - EK2285 |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 10ul |
| Sensitivity | 0.35 pg/ml |
| Standard Curve Range | 12.5 - 800 pg/ml |
| Spike Recovery Range | 91 % - 126 % |
| Mean Spike Recovery | 109% |
| CV of Intra plate | 3.0% - 3.5% |
| CV of Inter plate | 2.9% - 3.8% |
| Components |
96-well polystyrene microplate coated with a monoclonal antibody against FGF acidic Mouse FGF acidic Standard, lyophilized FGF acidic Detect Antibody Standard Diluent Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for mouse FGF acidic has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for FGF acidic are pipetted into the wells and FGF acidic present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, Streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of FGF acidic bound in the initial step. The color development is stopped and the intensity of the color is measured. |
