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Mouse FGF Acidic ELISA Kit

Mouse FGF Acidic ELISA Kit


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Product Details

Mouse FGF Acidic ELISA Kit

Brand MultiSciences
Product Code 70-EK2285 - 96
CatNum EK2285 - 96
Product Name Mouse FGF acidic ELISA Kit
Customs Name Mouse FGF acidic ELISA Kit
Type ELISA Kit
Application ELISA
Reactivity Mouse
SDS SDS - EK2285
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 10ul
Sensitivity 0.35 pg/ml 
Standard Curve Range 12.5 - 800 pg/ml
Spike Recovery Range 91 % - 126 % 
Mean Spike Recovery 109%
CV of Intra plate 3.0% - 3.5%
CV of Inter plate 2.9% - 3.8%

96-well polystyrene microplate coated with a monoclonal antibody against FGF acidic

Mouse FGF acidic Standard, lyophilized

FGF acidic Detect Antibody

Standard Diluent

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

Plate Covers


This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for mouse FGF acidic has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for FGF acidic are pipetted into the wells and FGF acidic present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, Streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of FGF acidic bound in the initial step. The color development is stopped and the intensity of the color is measured.