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Mouse IFN-γ ELISA Kit

Mouse IFN-γ ELISA Kit


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Mouse IFN-γ ELISA Kit

Product Code 70-EK280-96
CatNum EK280-96
Product Name Mouse IFN-γ ELISA Kit
Customs Name Mouse IFN-γ ELISA Kit
Product Spec 96T
Type ELISA Kit
Application ELISA
Reactivity Mouse
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 50ul
Sensitivity 0.83 pg/ml 
Standard Curve Range 23.44 - 1500 pg/ml
Spike Recovery Range 90 %-108 %
Mean Spike Recovery 101%
CV of Intra plate 4.6 % - 7.0 %
CV of Inter plate 3.2 % - 4.7 %

96-well polystyrene microplate coated with a monoclonal antibody against IFN-γ

Human IFN-γ Standard, lyophilized

IFN-γ Detect Antibody

Standard Diluent

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

Plate Covers


This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for mouse IFN-γ has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for IFN-γ are pipetted into the wells and IFN-γ present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of IFN-γ bound in the initial step. The color development is stopped and the intensity of the color is measured.