MULTI SCIENCES
Mouse IFN-γ ELISA Kit
Mouse IFN-γ ELISA Kit
SKU:EK280
Product Details
| Product Code | 70-EK280-96 |
|---|---|
| CatNum | EK280-96 |
| Product Name | Mouse IFN-γ ELISA Kit |
| Customs Name | Mouse IFN-γ ELISA Kit |
| Product Spec | 96T |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Mouse |
| SDS | SDS - EK280 |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 50ul |
| Sensitivity | 0.83 pg/ml |
| Standard Curve Range | 23.44 - 1500 pg/ml |
| Spike Recovery Range | 90 %-108 % |
| Mean Spike Recovery | 101% |
| CV of Intra plate | 4.6 % - 7.0 % |
| CV of Inter plate | 3.2 % - 4.7 % |
| Components |
96-well polystyrene microplate coated with a monoclonal antibody against IFN-γ Human IFN-γ Standard, lyophilized IFN-γ Detect Antibody Standard Diluent Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for mouse IFN-γ has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for IFN-γ are pipetted into the wells and IFN-γ present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of IFN-γ bound in the initial step. The color development is stopped and the intensity of the color is measured. |
