96-well polystyrene microplate coated with a monoclonal antibody against mouse Ig isotypes

Positive Control

Ig Isotyping Detect Antibody

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

Plate Covers

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse Ig Isotyping. The Mouse Ig Isotyping ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Antibody isotyping is a critical and beneficial aspect of hybridoma development. This kit can identify six immunoglobulin heavy chain isotypes and two light chain isotypes in mice: IgA, IgM, IgG1, IgG2a, IgG2b, IgG3, kappa chain and lambda chain. It can accurately and specifically identify which heavy and light chain of the hybridoma samples is producing and if it is monoclonal or not. This kit is a powerful tool to isolate and characterize each potential clone. 

Identification is essential since chemical and biological properties of the various classes are unique. They differ in their solubility and electrophoretic properties, susceptibility to cleavage enzymes, and reactivity with protein A. Determining the class and subclass of a monoclonal antibody is thus useful in planning the best immunoglobulin purification method. For example, mouse IgA and IgM are best purified by size (i.e., gel exclusion) or using immunoaffinity separation columns. Mouse IgG2a and IgG2b are purified with immobilized Protein A at pH 7 - 8, while Mouse IgG1 binds best to Protein A at pH 8 - 9. Immunoglobulin that contains kappa light chains can be purified using immobilized Protein L.