MULTI SCIENCES
Mouse IGFBP-2 ELISA Kit
Mouse IGFBP-2 ELISA Kit
SKU:EK2278
Product Details
| Factory Name | MultiSciences |
|---|---|
| Product Code | 70-EK2278-96 |
| CatNum | EK2278-96 |
| Product Name | Mouse IGFBP-2 ELISA Kit |
| Customs Name | Mouse SCF ELISA Kit |
| Product Spec | 96T |
| SalePrice | 3200 |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Mouse |
| Modality | NoData |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sensitivity | 6.13 pg/ml |
| Standard Curve Range | 93.75 - 6000 pg/ml |
| Spike Recovery Range | 88 %-103 % |
| Mean Spike Recovery | 94% |
| CV of Intra plate | 3.2 % - 4.6 % |
| CV of Inter plate | 3.7 % - 4.8 % |
| Components |
96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against mouse IGFBP-2 Mouse IGFBP-2 Standard, lyophilized IGFBP-2 Detect Antibody Streptavidin-HRP Assay Buffer (10×) Substrate Stop Solution Washing Buffer (20×) Adhesive Films |
| PRINCIPLE OF THE ASSAY | Mouse IGFBP-2 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse IGFBP-2 in the samples. An antibody specific for mouse IGFBP-2 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for IGFBP-2, and IGFBP-2 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IGFBP-2 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader. |
| Describtion | Insulin-like growth factor-binding proteins (IGFBPs) play an integral role in modifying insulin-like growth factor (IGF) actions in a wide variety of cell types. This family contains six members that are structurally related but encoded by distinct genes.IGFBP-2 is the second most abundant IGFBP in the circulation and is present in various other biological fluids and tissues of many vertebrate species. Serum IGFBP-2 levels are elevated in conditions such as shock, fasting, hypoxemia or after traumata, suggesting complex regulation of IGFBP-2 expression. IGFBP2 is overexpressed in many malignancies and is often correlated with an increasingly malignant status of the tumor, pointing to a potential involvement of IGFBP-2 in tumorigenesis.During development, IGFBP-2 is expressed in a number of tissues. The highest expression level is found in the central nervous system. In adults, high expression levels are also detected in the central nervous system and in a number of reproductive tissues. |
