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Factory Name MultiSciences
Product Code 70-EK2278-96
CatNum EK2278-96
Product Name Mouse IGFBP-2 ELISA Kit
Customs Name Mouse SCF ELISA Kit
Product Spec 96T
SalePrice 3200
Type ELISA Kit
Application ELISA
Reactivity Mouse
Modality NoData
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sensitivity 6.13 pg/ml
Standard Curve Range 93.75 - 6000 pg/ml
Spike Recovery Range 88 %-103 %
Mean Spike Recovery 94%
CV of Intra plate 3.2 % - 4.6 %
CV of Inter plate 3.7 % - 4.8 %

96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against mouse IGFBP-2

Mouse IGFBP-2 Standard, lyophilized

IGFBP-2 Detect Antibody


Assay Buffer (10×)


Stop Solution

Washing Buffer (20×)

Adhesive Films

PRINCIPLE OF THE ASSAY Mouse IGFBP-2 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse IGFBP-2 in the samples. An antibody specific for mouse IGFBP-2 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for IGFBP-2, and IGFBP-2 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IGFBP-2 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Insulin-like growth factor-binding proteins (IGFBPs) play an integral role in modifying insulin-like growth factor (IGF) actions in a wide variety of cell types. This family contains six members that are structurally related but encoded by distinct genes.IGFBP-2 is the second most abundant IGFBP in the circulation and is present in various other biological fluids and tissues of many vertebrate species. Serum IGFBP-2 levels are elevated in conditions such as shock, fasting, hypoxemia or after traumata, suggesting complex regulation of IGFBP-2 expression. IGFBP2 is overexpressed in many malignancies and is often correlated with an increasingly malignant status of the tumor, pointing to a potential involvement of IGFBP-2 in tumorigenesis.During development, IGFBP-2 is expressed in a number of tissues. The highest expression level is found in the central nervous system. In adults, high expression levels are also detected in the central nervous system and in a number of reproductive tissues.