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MULTI SCIENCES

Mouse IgM ELISA Kit Plate

Mouse IgM ELISA Kit Plate

SKU:EK276

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Mouse IgM ELISA Kit Plate

CatNum 70-EK276
Product Name Mouse IgM ELISA Kit
Customs Name Mouse IgM ELISA Kit
Application ELISA
Reactivity Mouse
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 10 μl (prediluted)
Sensitivity 19.26 pg/ml
Standard Curve Range 0.50 - 32 ng/ml
Spike Recovery Range 91%-107%
Mean Spike Recovery 102%
CV of Intra plate 3.2 % - 4.3 %
CV of Inter plate 2.9 % - 7.6 %
Components 96-well polystyrene microplate coated with a monoclonal antibody against IgM
Mouse IgM Standard, lyophilized
IgM Detect Antibody
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
Describtion This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse IgM. The Mouse IgM ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Immunoglobulin M (IgM) is a basic antibody that is produced by B cells. It is the first antibody to
appear in response to initial exposure to an antigen. The spleen, where plasmablasts responsible for antibody production reside, is the major site of specific IgM production. IgM in normal serum is often found to bind to specific antigens, even in the absence of prior immunization. For this reason IgM has sometimes been called a "natural antibody". Demonstrating IgM antibodies in a patient's serum indicates recent infection, or in a neonate's serum indicates intrauterine infection.