MULTI SCIENCES
Mouse IL-10 ELISA Kit Distributor
Mouse IL-10 ELISA Kit Distributor
SKU:EK210
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK210 |
| Product Name | Mouse IL-10 ELISA Kit |
| Customs Name | Mouse IL-10 ELISA Kit |
| Application | ELISA |
| Reactivity | Mouse |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 20 μl |
| Sensitivity | 4.8 pg/ml |
| Standard Curve Range | 125.00 - 8000 pg/ml |
| Spike Recovery Range | 86 % - 108% |
| Mean Spike Recovery | 0.97 |
| CV of Intra plate | 5.3 % - 7.1 % |
| CV of Inter plate | 3.8 % - 8.8 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against Mouse IL-10 Mouse IL-10 Standard, lyophilized Mouse IL-10 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse IL-10. The Mouse IL-10 ELISA is for research use only. Not for diagnostic or therapeutic procedures. Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 α-helical cytokine family that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155. Mature mouse IL-10 shares 85% amino acid sequence identity with rat IL-10 and 70-77% with bovine, canine, equine, feline, human, ovine, and porcine IL-10. It contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently-associated homodimer. IL-10 is primarily produced by monocytes and, to a lesser extent, lymphocytes, namely type 2 T helper cells (Th2), mastocytes, CD4+CD25+Foxp3+ regulatory T cells, and in a certain subset of activated T cells and B cells. IL-10 is a cytokine with multiple, pleiotropic, effects in immunoregulation and inflammation. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations. IL-10 can furthermore exert strong anti-inflammatory activities. |
