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Mouse IL-12p70 ELISA Kit

Mouse IL-12p70 ELISA Kit


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Product Details

Mouse IL-12p70 ELISA Kit

Brand MultiSciences
Product Code 70-EK212
CatNum EK2122
Product Name Mouse IL-12p70 ELISA Kit
Product Spec 96 T
Type ELISA Kit
Application ELISA
Reactivity Mouse
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 20 μl
Sensitivity 3.58pg/ml
Standard Curve Range 31.25 - 2000 pg/ml
Spike Recovery Range 89 % - 114 %
Mean Spike Recovery 99%
CV of Intra plate 5.2 % - 9.4 %
CV of Inter plate 6.5 % - 7.9 %

96-well polystyrene microplate coated with a monoclonal antibody against IL-12p70

Human IL-12p70 Standard, lyophilized

IL-12p70 Detect Antibody

Standard Diluent


Amplification Reagent Concentrate

Amplification Diluent

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

washing Buffer (20×)

Plate Covers

Describtion This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for mouse IL-12p70 has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for IL-12p70 are pipetted into the wells and IL-12p70 present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of IL-12p70 bound in the initial step. The color development is stopped and the intensity of the color is measured.