MULTI SCIENCES
Mouse IL-12p70 High Sensitivity ELISA Kit
Mouse IL-12p70 High Sensitivity ELISA Kit
SKU:EK212HS
Product Details
| Factory Name | MultiSciences |
|---|---|
| Product Code | 70-EK212HS-96 |
| CatNum | EK212HS-96 |
| Product Name | Mouse IL-12p70 High Sensitivity ELISA Kit |
| Product Spec | 96 T |
| SalePrice | 3200 |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Mouse |
| Modality | No Data |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sensitivity | 0.57 pg/ml |
| Standard Curve Range | 7.81- 500 pg/ml |
| Spike Recovery Range | 92 %-115 % |
| Mean Spike Recovery | 104% |
| CV of Intra plate | 4.6 % - 5.5 % |
| CV of Inter plate | 2.6 % - 3.1 % |
| Components |
96-well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody against rat VEGF Rat VEGF HS Standard, lyophilized IL-12p70 HS Detect Antibody Standard Diluent Streptavidin-HRP Amplification Reagent Concentrate Amplification Diluent Assay Buffer (10×) Substrate Stop Solution Washing Buffer (20×) Plate Covers |
| PRINCIPLE OF THE ASSAY | Mouse IL-12p70 High Sensitivity ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse IL-12p70 in the samples. A monoclonal antibody specific for mouse IL-12p70 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and IL-12p70 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for IL-12p70 is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, amplification reagent is added to the wells. Following incubation any unbound substances is removed during a wash step and streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of IL-12p70 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Describtion |
Interleukin 12 (IL-12) is an interleukin that is naturally produced by dendritic cells, macrophages, neutrophils human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is composed of a bundle of four alpha helices. It is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). The active heterodimer (referred to as 'p70'), and a homodimer of p40 are formed following protein synthesis. IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. IL-12 has anti-angiogenic activity, and plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 is linked with autoimmunity as well. |
