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Mouse IL-12p70 High Sensitivity ELISA Kit

Mouse IL-12p70 High Sensitivity ELISA Kit


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Mouse IL-12p70 High Sensitivity ELISA Kit

Factory Name MultiSciences
Product Code 70-EK212HS-96
CatNum EK212HS-96
Product Name Mouse IL-12p70 High Sensitivity ELISA Kit
Product Spec 96 T
SalePrice 3200
Type ELISA Kit
Application ELISA
Reactivity Mouse
Modality No Data
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sensitivity 0.57 pg/ml
Standard Curve Range 7.81- 500 pg/ml
Spike Recovery Range 92 %-115 %
Mean Spike Recovery 104%
CV of Intra plate 4.6 % - 5.5 %
CV of Inter plate 2.6 % - 3.1 %

96-well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody against rat VEGF

Rat VEGF HS Standard, lyophilized

IL-12p70 HS Detect Antibody

Standard Diluent


Amplification Reagent Concentrate

Amplification Diluent                                                                                   

Assay Buffer (10×)


Stop Solution

Washing Buffer (20×)

Plate Covers

PRINCIPLE OF THE ASSAY Mouse IL-12p70 High Sensitivity ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse IL-12p70 in the samples. A monoclonal antibody specific for mouse IL-12p70 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and IL-12p70 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for IL-12p70 is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, amplification reagent is added to the wells. Following incubation any unbound substances is removed during a wash step and streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of IL-12p70 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Interleukin 12 (IL-12) is an interleukin that is naturally produced by dendritic cells, macrophages, neutrophils human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is composed of a bundle of four alpha helices. It is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). The active heterodimer (referred to as 'p70'), and a homodimer of p40 are formed following protein synthesis.

IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. IL-12 has anti-angiogenic activity, and plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 is linked with autoimmunity as well.