MULTI SCIENCES
Mouse IL-15/IL-15R Sandwich ELISA Kit
Mouse IL-15/IL-15R Sandwich ELISA Kit
SKU:EK215R
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK215R |
| Product Name | Mouse IL-15/IL-15R ELISA Kit |
| Customs Name | Mouse IL-15/IL-15R ELISA Kit |
| Application | ELISA |
| Reactivity | Mouse |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 20 μl |
| Sensitivity | 0.65 pg/ml |
| Standard Curve Range | 7.81 - 500 pg/ml |
| Spike Recovery Range | 88 % - 112 % |
| Mean Spike Recovery | 1.01 |
| CV of Intra plate | 2.4 % - 4.5 % |
| CV of Inter plate | 3.0 % - 6.2 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against IL-15/IL-15R Mouse IL-15/IL-15R Standard, lyophilized IL-15/IL-15R Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse IL-15/IL-15R. The Mouse IL-15/IL-15R ELISA is for research use only. Not for diagnostic or therapeutic procedures. Interleukin-15 (IL-15) is a proinflammatory protein that has been shown to play a role in the activation of neutrophils, dendritic cells and macrophages, and is essential to the development and survival of NK cells and CD8 T-cells. IL-15 activates Jak/Stat pathways by signaling through a heterotrimeric receptor. Despite the expression of IL-15 mRNA in many cell types, the secreted protein is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and is only secreted in complex with its unique receptor, IL-15R alpha. The soluble IL-15/IL-15R complex is produced by mouse dendritic cells, fibroblasts, and macrophages, and demonstrates a 10-100-fold increase in agonistic activity over IL-15 alone. |
