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Mouse IL-17AF Enzyme Immunoassay Kit

Mouse IL-17AF Enzyme Immunoassay Kit


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Product Details

Mouse IL-17AF  Enzyme Immunoassay Kit

Brand MultiSciences
CatNum 70-EK21772
Product Name Mouse IL-17AF ELISA Kit
Customs Name Mouse IL-17AF ELISA Kit
Application ELISA
Reactivity Mouse
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 20 μl
Sensitivity 4.54 pg/ml
Standard Curve Range 46.88 - 3000 pg/ml
Spike Recovery Range 73 % - 125 %
Mean Spike Recovery 1.1
CV of Intra plate 4.4 % - 7.7 %
CV of Inter plate 4.7 % - 6.8 %
Components 96-well polystyrene microplate coated with a monoclonal antibody against IL-17AF
Mouse IL-17AF Standard, lyophilized
IL-17AF Detect Antibody
Standard Diluent
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
Describtion This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse IL-17AF. The Mouse IL-17AF ELISA is for research use only. Not for diagnostic or therapeutic procedures.
IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. Of the six IL-17 family members, IL-17A and IL-17F share the strongest homology (50% amino acid identity) and the two genes are located in the same chromosomal region. IL-17A and IL-17F have been observed in tissue samples from various autoimmune diseases, such as rheumatoid arthritis, multiple sclerosis, psoriasis, inflammatory bowel disease, and asthma.
IL-17A expression is associated with many inflammatory diseases in humans, such as rheumatoid arthritis, multiple sclerosis, asthma, systemic lupus erythematosus, and allograft rejection. Expression of IL-17F is also linked with human inflammatory diseases, including asthma.