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Mouse IL-1β ELISA Kit

Mouse IL-1β ELISA Kit


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Product Details

Mouse IL-1β ELISA Kit

Brand MultiSciences
Product Code 70-EK201B2/2
CatNum EK201B2/2
Product Name Mouse IL-1β ELISA Kit
Product Spec 96 T
Type ELISA Kit
Application ELISA
Reactivity Mouse
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 10 μl
Sensitivity 1.45pg/ml
Standard Curve Range 23.44 - 1500 pg/ml
Spike Recovery Range 83 % - 109 %
Mean Spike Recovery 97%
CV of Intra plate 2.8 % - 5.6 %
CV of Inter plate 4.2 % - 8.4 %

96-well polystyrene microplate coated with a monoclonal antibody against IL-1β

Human IL-1β Standard, lyophilized

IL-1β Detect Antibody

Standard Diluent


Amplification Reagent Concentrate

Amplification Diluent

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

washing Buffer (20×)

Plate Covers

Describtion This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for mouse IL-1β has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for IL-1β are pipetted into the wells and IL-1β present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of IL-1β bound in the initial step. The color development is stopped and the intensity of the color is measured.