MULTI SCIENCES
Mouse IL-1β ELISA Kit
Mouse IL-1β ELISA Kit
SKU:EK201B2
Product Details
| Brand | MultiSciences |
|---|---|
| Product Code | 70-EK201B2/2 |
| CatNum | EK201B2/2 |
| Product Name | Mouse IL-1β ELISA Kit |
| Product Spec | 96 T |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Mouse |
| SDS | SDS - EK201B |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 10 μl |
| Sensitivity | 1.45pg/ml |
| Standard Curve Range | 23.44 - 1500 pg/ml |
| Spike Recovery Range | 83 % - 109 % |
| Mean Spike Recovery | 97% |
| CV of Intra plate | 2.8 % - 5.6 % |
| CV of Inter plate | 4.2 % - 8.4 % |
| Components |
96-well polystyrene microplate coated with a monoclonal antibody against IL-1β Human IL-1β Standard, lyophilized IL-1β Detect Antibody Standard Diluent Streptavidin-HRP Amplification Reagent Concentrate Amplification Diluent Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for mouse IL-1β has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for IL-1β are pipetted into the wells and IL-1β present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of IL-1β bound in the initial step. The color development is stopped and the intensity of the color is measured. |
