MULTI SCIENCES
Mouse IL-1β Pro-form ELISA Kit Plate
Mouse IL-1β Pro-form ELISA Kit Plate
SKU:EK2195
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK2195 |
| Product Name | Mouse IL-1β Pro-form ELISA Kit |
| Customs Name | Mouse IL-1β Pro-form ELISA Kit |
| Application | ELISA |
| Reactivity | Mouse |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 20 μl |
| Sensitivity | 1.98 pg/ml |
| Standard Curve Range | 46.88 - 3000 pg/ml |
| Spike Recovery Range | 83 % - 111 % |
| Mean Spike Recovery | 0.97 |
| CV of Intra plate | 1.9 % - 4.4 % |
| CV of Inter plate | 3.3 % - 4.1 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against IL-1β Pro-form Mouse IL-1β Pro-form Standard, lyophilized IL-1β Pro-form Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse IL-1β Pro-form. The Mouse IL-1β Pro-form ELISA is for research use only. Not for diagnostic or therapeutic procedures. Interleukin-1 beta (IL-1β) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. It is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1β into the active 17 kDa protein requires the activation of inflammasomes. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1β. Although IL-1β is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1β signals through two receptors, IL-1RI and IL-1RII. These cytokines play important roles in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. Their activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. Elevated levels of IL-1β have been associated with many chronic inflammatory conditions, giving IL-RA or IL-1β neutralizing antibodies potential therapeutical value. |
