MULTI SCIENCES
Mouse IL-1ra/IL-1F3 ELISA Kit
Mouse IL-1ra/IL-1F3 ELISA Kit
SKU:EK2132
Product Details
| Factory Name | MultiSciences |
|---|---|
| Product Code | 70-EK2132-96 |
| CatNum | EK2132-96 |
| Product Name | Mouse IL-1ra/IL-1F3 ELISA Kit |
| Customs Name | Mouse IL-1ra/IL-1F3 ELISA Kit |
| Product Spec | 96T |
| SalePrice | 3200 |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Mouse |
| Modality | NoData |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sensitivity | 0.70 pg/ml |
| Standard Curve Range | 31.25 - 2000 pg/ml |
| Spike Recovery Range | 88 %-109 % |
| Mean Spike Recovery | 93% |
| CV of Intra plate | 3.5 % - 4.3 % |
| CV of Inter plate | 4.5 % - 4.9 % |
| Components |
96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against mouse IL-1ra Mouse IL-1ra Standard, lyophilized IL-1ra Detect Antibody Streptavidin-HRP Assay Buffer (10×) Substrate Stop Solution Washing Buffer (20×) Adhesive Films |
| PRINCIPLE OF THE ASSAY | Mouse IL-1ra/IL-1F3 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse IL-1ra in the samples. An antibody specific for mouse IL-1ra has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and IL-1ra present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for IL-1ra is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of IL-1ra bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Describtion | Interleukin-1 receptor antagonist (IL-1ra, also known as IL-1F3) is a member of the IL-1 cytokine family of proteins that includes IL-1α and IL-1β. IL-1ra inhibits the activity of interleukin-1 by binding to receptor IL-1R1 and preventing its association with the coreceptor IL-1RAP for signaling. Mouse Interleukin-1 receptor antagonist (IL-1ra) is a 22-25 kDa glycoprotein produced by a variety of cell types. Cells known to secrete IL-1ra include dermal fibroblasts, vascular smooth muscle cells, intestinal columnar epithelium, chondrocytes, macrophages, non-keratinized oral stratified squamous epithelium, mast cells, neutrophils and monocytes, Sertoli cells, and hepatocytes. There are two type I transmembrane glycoprotein receptors for IL-1ra: the bioactive 80 kDa type I IL-1 receptor (IL-1 RI), and the inert (decoy) 65 kDa type II IL-1 receptor (IL-1 RII). IL-1ra blocks the proinflammatory actions of IL-1 including Prostaglandin E2 and IL-6 release from endothelial cells, fever generation, thrombocytosis, the production of hepatic acute phase proteins, and neutrophil expansion and infiltration. |
