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Mouse IL-1ra/IL-1F3 ELISA Kit

Mouse IL-1ra/IL-1F3 ELISA Kit


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Mouse IL-1ra/IL-1F3 ELISA Kit

Factory Name MultiSciences
Product Code 70-EK2132-96
CatNum EK2132-96
Product Name Mouse IL-1ra/IL-1F3 ELISA Kit
Customs Name Mouse IL-1ra/IL-1F3 ELISA Kit
Product Spec 96T
SalePrice 3200
Type ELISA Kit
Application ELISA
Reactivity Mouse
Modality NoData
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sensitivity 0.70 pg/ml
Standard Curve Range 31.25 - 2000 pg/ml
Spike Recovery Range 88 %-109 %
Mean Spike Recovery 93%
CV of Intra plate 3.5 % - 4.3 %
CV of Inter plate 4.5 % - 4.9 %

96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against mouse IL-1ra

Mouse IL-1ra Standard, lyophilized

IL-1ra Detect Antibody


Assay Buffer (10×)


Stop Solution

Washing Buffer (20×)

Adhesive Films

PRINCIPLE OF THE ASSAY Mouse IL-1ra/IL-1F3 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse IL-1ra in the samples. An antibody specific for mouse IL-1ra has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and IL-1ra present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for IL-1ra is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of IL-1ra bound in the initial step. The color development is stopped and the intensity of the color is measured.

Interleukin-1 receptor antagonist (IL-1ra, also known as IL-1F3) is a member of the IL-1 cytokine family of proteins that includes IL-1α and IL-1β. IL-1ra inhibits the activity of interleukin-1 by binding to receptor IL-1R1 and preventing its association with the coreceptor IL-1RAP for signaling. Mouse Interleukin-1 receptor antagonist (IL-1ra) is a 22-25 kDa glycoprotein produced by a variety of cell types. Cells known to secrete IL-1ra include dermal fibroblasts, vascular smooth muscle cells, intestinal columnar epithelium, chondrocytes, macrophages, non-keratinized oral stratified squamous epithelium, mast cells, neutrophils and monocytes, Sertoli cells, and hepatocytes. There are two type I transmembrane glycoprotein receptors for IL-1ra: the bioactive 80 kDa type I IL-1 receptor (IL-1 RI), and the inert (decoy) 65 kDa type II IL-1 receptor (IL-1 RII). IL-1ra blocks the proinflammatory actions of IL-1 including Prostaglandin E2 and IL-6 release from endothelial cells, fever generation, thrombocytosis, the production of hepatic acute phase proteins, and neutrophil expansion and infiltration.