MULTI SCIENCES
Mouse IL-22 Enzyme Immunoassay Kit
Mouse IL-22 Enzyme Immunoassay Kit
SKU:EK222
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK222 |
| Product Name | Mouse IL-22 ELISA Kit |
| Customs Name | Mouse IL-22 ELISA Kit |
| Application | ELISA |
| Reactivity | Mouse |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 20 μl |
| Sensitivity | 0.74 pg/ml |
| Standard Curve Range | 15.63 - 1000 pg/ml |
| Spike Recovery Range | 85 % - 118 % |
| Mean Spike Recovery | 0.94 |
| CV of Intra plate | 2.9 % - 7.9 % |
| CV of Inter plate | 3.1 % - 5.4 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against IL-22 Mouse IL-22 Standard, lyophilized IL-22 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse IL-22. The Mouse IL-22 ELISA is for research use only. Not for diagnostic or therapeutic procedures. IL-22 is an α-helical cytokine. IL-22 binds to a heterodimeric cell surface receptor composed of IL-10R2 and IL-22R1 subunits.IL-22R is expressed on tissue cells, and it is absent on immune cells.In the mouse, the IL-22 gene is located on chromosome 10, in the same region as the IFN gamma gene. IL-22, in contrast to its relative IFN gamma, regulates the expression of only a few genes in keratinocytes. This is due to varied signal transduction. IL-22 biological activity is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). IL-22 and IL-10 receptor chains play a role in cellular targeting and signal transduction to selectively initiate and regulate immune responses. IL-22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. IL-22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10. |
