MULTI SCIENCES
Mouse IL-33 Protein A ELISA Kit
Mouse IL-33 Protein A ELISA Kit
SKU:EK233
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK233 |
| Product Name | Mouse IL-33 ELISA Kit |
| Customs Name | Mouse IL-33 ELISA Kit |
| Application | ELISA |
| Reactivity | Mouse |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 20 μl |
| Sensitivity | 8.74 pg/ml |
| Standard Curve Range | 46.88 - 3000 pg/ml |
| Spike Recovery Range | 85 % - 117 % |
| Mean Spike Recovery | 0.98 |
| CV of Intra plate | 4.9 % - 8.9 % |
| CV of Inter plate | 4.9 % - 8.1 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against IL-33 Mouse IL-33 Standard, lyophilized IL-33 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse IL-33. The Mouse IL-33 ELISA is for research use only. Not for diagnostic or therapeutic procedures. Interleukin 33 (IL-33), also known as NF-HEV and DVS 27, is a 30-kDa member of the IL-1 family produced by a wide variety of cell types, including fibroblasts, mast cells, dendritic cells, macrophages, osteoblasts, endothelial cells, and epithelial cells. IL-33 induces helper T cells, mast cells, eosinophils and basophils to produce type 2 cytokines. IL-33 mediates its biological effects by interacting with the receptors ST2 and IL-1 Receptor Accessory Protein (IL1RAP), activating intracellular molecules in the NF-κB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-5 and IL-13) from polarized Th2 cells. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. |
