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Mouse IL-5 ELISA Assay Kit

Mouse IL-5 ELISA Assay Kit


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Product Details

Mouse IL-5 ELISA Assay Kit

Brand MultiSciences
CatNum 70-EK205
Product Name Mouse IL-5 ELISA Kit
Customs Name Mouse IL-5 ELISA Kit
Application ELISA
Reactivity Mouse
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 10 μl
Sensitivity 3.14 pg/ml
Standard Curve Range 15.63 - 1000 pg/ml
Spike Recovery Range 80 % - 118 %
Mean Spike Recovery 100%
CV of Intra plate 2.7 % - 4.2 %
CV of Inter plate 2.5 % - 4.2 %
Components 96-well polystyrene microplate coated with a monoclonal antibody against Dkk-1
Mouse Dkk-1 Standard, lyophilized
Dkk-1 Detect Antibody
Standard Diluent
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse IL-5. The Mouse IL-5 ELISA is for research use only. Not for diagnostic or therapeutic procedures. IL-5 is a 115-amino acid-long Th2 cytokine that is part of the hematopoietic family. Unlike other members of this cytokine family (namely IL-3 and GM-CSF), this glycoprotein in its active form is a homodimer. IL-5 is produced by Th2 cells and mast cells. The spectrum of biologicalactivities of IL-5, on B cells as well as other cell types suggests that IL-5 plays an important functional role in the activities of Th2 cells. Through binding to the IL-5 receptor, IL-5 stimulates B cell growth and increases immunoglobulin secretion. It is also a key mediator in eosinophil activation.

IL-5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma, wherein a large increase in the number of circulating, airway tissue, and induced sputum eosinophils have been observed.