MULTI SCIENCES
Mouse LIF Sandwich ELISA Kit
Mouse LIF Sandwich ELISA Kit
SKU:EK2107
Product Details
| Factory Name | MultiSciences |
|---|---|
| CatNum | EK2107-96 |
| Product Name | Mouse LIF ELISA Kit |
| Customs Name | Mouse LIF ELISA Kit |
| Product Spec | 96T |
| Application | ELISA |
| Reactivity | Mouse |
| SDS | SDS - EK2107 |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 50 μl (prediluted) |
| Sensitivity | 0.77 pg/ml |
| Standard Curve Range | 7.81 - 500 pg/ml |
| Spike Recovery Range | 91 % - 120% |
| Mean Spike Recovery | 108% |
| CV of Intra plate | 3.8 % - 4.8 % |
| CV of Inter plate | 3.9 % - 5.9 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against LIF Mouse LIF Standard, lyophilized LIF Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse LIF. The Mouse LIF ELISA is for research use only. Not for diagnostic or therapeutic procedures. Leukemia inhibitory factor (LIF) is an interleukin 6 family cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. Cells known to express LIF include activated T-cells, monocytes, and astrocytes, osteoblasts, keratinocytes, mast cells, and fibroblasts. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. During pregnancy LIF is involved in decidualization of the maternal endometrium and implantation of the blastocyst to the endometrium. Women with decreased production of LIF and other cytokines are fertile and able to become pregnant, but there is an increased risk for unexplained, recurrent miscarriages. It has been suggested that recombinant human LIF might help to improve the implantation rate in women with unexplained infertility. In addition, LIF is typically added to stem cell culture medium to reduce spontaneous differentiation. |
